Spatial and temporal resolution in cryo-electron microscopy—A scope for nano-chemistry

Defects and Structural Analysis of Multi-Wall Carbon Nano Tubes via Ball milling and Cryo-milling

Nano fillers are part of new studies to enhance various properties of fluids and solids. For example, adding nanoparticles as an enforcement in nanocomposites or as an additive to improve thermal or electrical properties in nano-fluids are of extreme importance in science and industry. There are numerous methods to uniformly disperse nanoparticles in fluid and solid phase. One of the well-used ...

Mathematics for cryo - electron microscopy

Single-particle cryo-electron microscopy (cryo-EM) has recently joined X-ray crystallography and NMR spectroscopy as a high-resolution structural method for biological macromolecules. Cryo-EM was selected by Nature Methods as Method of the Year 2015, large scale investments in cryo-EM facilities are being made all over the world, and the Nobel Prize in Chemistry 2017 was awarded to Jacques Dubo...

Correlative cryo-fluorescence light microscopy and cryo-electron tomography of Streptomyces.

Light microscopy and electron microscopy are complementary techniques that in a correlative approach enable identification and targeting of fluorescently labeled structures in situ for three-dimensional imaging at nanometer resolution. Correlative imaging allows electron microscopic images to be positioned in a broader temporal and spatial context. We employed cryo-correlative light and electro...

“ for developing cryo - electron microscopy for the high - resolution structure determination of biomolecules in solution ” 2017 Nobel Prize in Chemistry

Anisotropic Correction of Beam-induced Motion for Improved Single-particle Electron Cryo-microscopy

Correction of electron beam-induced sample motion is one of the major factors contributing to the recent resolution breakthroughs in cryo-electron microscopy. Improving the accuracy and efficiency of motion correction can lead to further resolution improvement. Based on observations that the electron beam induces doming of the thin vitreous ice layer, we developed an algorithm to correct anisot...